mos1-mediated Single Copy Insertion
MosSCI, Mos1-mediated single-copy insertion, utilizes the Mos1 transposon to produce a double-stranded break in DNA, generate single copy insertion through homologous recombination repair. Even the CRISPR/Cas9 gene editing system provides enhanced processing, MosSCI still remains a good approach for the insertion of large transgenes without affecting the original C.elegans genes.
MosSCI allows us to insert a transgene in single copy at several defined sites allowing gene expression in a broad range of tissues at apparently endogenous level.
Figure 1 Microinject DNA to generate array
Figure 2 Activate Mos1 transposase and excise Mos1
Figure 3 Gene conversion off template
Figure 4 Loose array and recover insertion
|G001-MosSCI||Starts from 1400 USD*||6-8|
Information of both target plasmid and background strain
*Price is determined by the insert length.
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