Mos1-mediated Single Copy Insertion
MosSCI, Mos1-mediated single-copy insertion, utilizes the Mos1 transposon to produce a double-stranded break in DNA, generate single copy insertion through homologous recombination repair. Even the CRISPR/Cas9 gene editing system provides enhanced processing, MosSCI still remains a good approach for the insertion of large transgenes without affecting the original C.elegans genes.
MosSCI allows us to insert a transgene in single copy at several defined sites allowing gene expression in a broad range of tissues at apparently endogenous level.
Figure 1 Microinject DNA to generate array
Figure 2 Activate Mos1 transposase and excise Mos1
Figure 3 Gene conversion off template
Figure 4 Loose array and recover insertion
| Product | Price | Turnaround(weeks) | Materials | Deliverables |
|---|---|---|---|---|
| G001-MosSCI | Starts from 2100 USD* | 6-8 | Information of both target plasmid and background strain |
|
*Price is determined by the insert length.
How to Order:
Download the order form OR Start your inquiry
Have Other Questions?
You can always reach us at service@sunybiotech.com. Our customer service and editing experts will get back to you ASAP.
