MosSCI
MosSCI
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mos1-mediated Single Copy Insertion

MosSCI, Mos1-mediated single-copy insertion, utilizes the Mos1 transposon to produce a double-stranded break in DNA, generate single copy insertion through homologous recombination repair. Even the CRISPR/Cas9 gene editing system provides enhanced processing, MosSCI still remains a good approach for the insertion of large transgenes without affecting the original C.elegans genes.

MosSCI allows us to insert a transgene in single copy at several defined sites allowing gene expression in a broad range of tissues at apparently endogenous level.

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Figure 1    Microinject DNA to generate array

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Figure 2    Activate Mos1 transposase and excise Mos1

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Figure 3    Gene conversion off template

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Figure 4   Loose array and recover insertion

ProductPriceTurnaround(weeks)MaterialsDeliverables
G001-MosSCIStarts from 1400 USD*6-8

Information of both target plasmid and background strain

  • 1. Mos1 insertion lines
  • 2. Sequencing report

  • 3. Genotyping figure

*Price is determined by the insert length.

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