Both MosSCI and miniMos are methods to insert single cope transgenes into genome of C. elegans. Both methods utilize the Mos1 transposase to produce a double-strand break in genomic DNA, which is repaired using your target plasmid as a template. Although the CRISPR/Cas9 gene editing system provides enhanced processing, MosSCI and miniMos still remain good approaches for the single copy insertion of large transgenes without the influence of the original genes.
Both MosSCI and miniMos are usually used to insert exogenous or endogenous translation constructs( with epitope tags or fluorescent tags) into the C.elegans genome.
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