CRISPR/Cas9 Yeast Gene Editing Service
Thanks to the convenient CRISPR/Cas9 gene editing system, gene editing in yeast, C. elegans, Drosophila, zebrafish, mice, human cells, and other species more efficient. Currently, we have established a complete CRISPR/Cas9-mediated guided yeast gene editing system (Figure 1) to rapidly and efficiently perform the following edits: precise sequence knockouts, precise single-base mutations, precise sequence insertions, precise short protein tag insertions, and precise sequence replacements.
Figure 1 CRISPR/Cas9 Gene Editing System
Saccharomyces cerevisiae has wide applications in both basic and applied research.
In basic research:
- 1.Genomics research: Saccharomyces cerevisiae has become an ideal model organism for studying gene expression, gene function, and gene family relationships.
- 2.Biochemical research: Saccharomyces cerevisiae can be used to study biochemical mechanisms including carbon metabolism, protein degradation, and cell cycle regulation.
- 3.Medical research: Saccharomyces cerevisiae can be used to study disease mechanisms such as protein aggregation and metabolic disorders.
In applied research:
- 1.Metabolic engineering: Modifying metabolic pathways of Saccharomyces cerevisiae to produce and synthesize industrial chemicals and biofuels, such as lactic acid, ethanol, butanol, etc.
- 2.Synthetic biology: Inserting the whole metabolic pathway to Saccharomyces cerevisiae de novo synthesis of biomaterials such as vitamins, antibiotics, proteins, etc.
Table 1 Service Process for Yeast Transgenic Technology
| Steps | Materials | Standard |
|---|---|---|
| Sample preparation | sgRNA plasmid, repair template | High-purity plasmid preparation |
| Yeast transformation | Specified yeast strain | High-qualityyeast competent cells |
| Preliminary screening | Single yeast clones on selective medium | Mutated yeast clone containing target gene editing |
| Molecular verification | Potential positive yeast clones with target editing | Mutated yeast clone with precise target gene editing verified by the sequencing |
Table 2 Prices for Yeast Gene Editing Services
| PID | Yeast gene editing | Turnaround time (weeks) | Price(US$)/strain | ||
|---|---|---|---|---|---|
| A202 | Precise gene deletion | 4-5 | 600 | ||
| A203 | Precise nucleotide changes | 4-5 | 650 | ||
| A204 | Precise sequence knock-in | 4-5 | 700 | ||
For cases involving sequence deletions larger than 1500 bp or insertions greater than 900 bp, please contact service@sunybiotech.com for quotation. | |||||
Deliverables:
- 1.Edited strains verified by sequencing
- 2.Sequencing report
How to Order:
Download the order form OR Start your inquiry
Have Other Questions?
You can always reach us at service@sunybiotech.com. Our customer service and editing experts will get back to you ASAP.
