Precise Sequence Replacement

Combination of the CRISPR/Cas9 sequence deletion and sequence knock-in technology allows researchers to make one-for-one substitution of DNA sequence, such as the promoter substitution, coding sequence substitution, gene swap.

Researchers can use this technology to study the function of both genomic genes and exogenous genes.

Gene replacement is the consequence of the combination of sequence deletion and sequence insertion.

  • 8.png
  • 7.png
  • 6.png
  • 5.png
  • 4.png
  • 3.png
  • 2.png
  • 1.png
  • 0.png

Figure 1 Schematic diagram of precise sequence replacement

PriceTurnaround(weeks)You need to provideDeliverables
Starts from 1699 USD*5-8
  1. 1. Name of gene on which you want to do precise gene replacement
  2. 2. Detailed sequence you want to delete

  3. 3. Detailed sequence you want to insert

  4. 4. Strain on which you want to do gene replacement
  1. 1. Homozygous animal with precise gene replacement**
  2. 2. Sequencing report

*The price depends on the deletion & insertion sizes and difficulties.

**If the precise nucleotide change leads to embryonic lethal, larva arrested, or sterile phenotype in homozygotes, the precise heterozygote becomes the deliverables. If you want balanced heterozygotes, we could provide a Balancer Cross Service at a price of 400 USD.

How to Order:

Download the order form    OR    Start your inquiry

Have Other Questions?


You can always reach us at Our customer service and editing experts will get back to you ASAP.