CRISPR/Cas9 Genome Edited C. elegans
CRISPR/Cas9 Genome Edited C. elegans

Gene Editing Services using the CRISPR/Cas9 System

We have established an efficient genome editing system in C. elegans using the CRISPR/Cas9 system(Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These sequence changes include precise gene deletion, precise nucleotide substitutions, precise sequence knock-in, and precise sequence replacement.

  • 8.png
  • 7.png
  • 6.png
  • 5.png
  • 4.png
  • 3.png
  • 2.png
  • 1.png
  • 0.png

Figure 1    Schematic diagram of gene editing via the CRISPR/Cas9 system

Procedures and Prices of Services:

Table 1 Procedures of CRISPR/Cas9 gene editing services

ProceduresMaterialsStandard
Reagent preparationsgRNA plasmids, Cas9 plasmid and repair templatesHigh quality plasmids and templates
MicroinjectionThe designated C. elegans strains10-30 adults
ScreeningF1 and/or F2 progeny of injected wormsAnimals with the desired editing
Final verificationAnimals containing the desired sequence changesVerification by sequencing

Table 2 Price of CRISPR/Cas9 gene editing services

PIDGene editing using the CRISPR/Cas9 systemTurnaround(weeks)Price(US$)/strain
A0002.PNGPrecise sequence/gene deletion(≤1500bp)*5-8from 899 USD
A0003.PNGPrecise nucleotide changes5-8from 899 USD
A0004.PNGPrecise sequence knock-in(≤900bp)*5-8from 1399 USD
A0006.PNGPrecise sequence replacement(deletion≤1500bp)(insertion≤900bp)*5-8from 1499 USD
*: Please contact us for a quote if the edited sequence size is larger than the number in the price list.

Deliverables:

  1. 1.Edited strains verified by sequencing
  2. 2.Sequencing report

How to Order:

To get a quote or place an order, please register and login your account, download and fill out the order form(s) of the service(s) you need, and email the form(s) to service@sunybiotech.com. We will send the confirmed order form with a quote to your email address (the one related to your account) after checking the information.

Order Now

Have Other Questions?

FAQs

You can always reach us at service@sunybiotech.com. Our customer service and editing experts will get back to you ASAP.