CRISPR/Cas9 Genome Edited C. elegans
CRISPR/Cas9 Genome Edited C. elegans

Gene Editing Services using the CRISPR/Cas9 System

We have established an efficient genome editing system in C. elegans using the CRISPR/Cas9 system(Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These sequence changes include precise gene deletion, precise nucleotide substitutions, precise sequence knock-in, and precise sequence replacement.

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Figure 1    Schematic diagram of gene editing via the CRISPR/Cas9 system

Procedures and Prices of Services:

Table 1 Procedures of CRISPR/Cas9 gene editing services

ProceduresMaterialsStandard
Reagent preparationsgRNA plasmids, Cas9 plasmid and repair templatesHigh quality plasmids and templates
MicroinjectionThe designated C. elegans strains10-30 adults
ScreeningF1 and/or F2 progeny of injected wormsAnimals with the desired editing
Final verificationAnimals containing the desired sequence changesVerification by sequencing

Table 2 Price of CRISPR/Cas9 gene editing services

PIDGene editing using the CRISPR/Cas9 systemTurnaround(weeks)Price(US$)/strain
A0002.PNGPrecise sequence/gene deletion(≤1500bp)*5-8from 899 USD
A0003.PNGPrecise nucleotide changes5-8from 1049 USD
A0004.PNGPrecise sequence knock-in(≤100bp)*5-8from 1399 USD
A0006.PNGPrecise sequence replacement (deletion≤1500bp) (insertion≤100bp)*5-8from 1499 USD

* Please contact us for a quote if the edited sequence size is larger than the number in the price list.

Deliverables:

  1. 1.Edited strains verified by sequencing
  2. 2.Sequencing report

How to Order:

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Have Other Questions?

FAQs

You can always reach us at service@sunybiotech.com. Our customer service and editing experts will get back to you ASAP.