Gene Editing Services using the CRISPR/Cas9 System
We have established an efficient genome editing system in C. elegans using the CRISPR/Cas9 system(Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These sequence changes include precise gene deletion, precise nucleotide substitutions, precise sequence knock-in, and precise sequence replacement.
Figure 1 Schematic diagram of gene editing via the CRISPR/Cas9 system
Procedures and Prices of Services:
Table 1 Procedures of CRISPR/Cas9 gene editing services
|Reagent preparation||sgRNA plasmids, Cas9 plasmid and repair templates||High quality plasmids and templates|
|Microinjection||The designated C. elegans strains||10-30 adults|
|Screening||F1 and/or F2 progeny of injected worms||Animals with the desired editing|
|Final verification||Animals containing the desired sequence changes||Verification by sequencing|
Table 2 Price of CRISPR/Cas9 gene editing services
- 1.Edited strains verified by sequencing
- 2.Sequencing report
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