Precise sequence knock-in
CRISPR/Cas9 sequence knock-in technology allows researchers to insert any sequence they want, such as GFP, mCherry, wrmScarlet, FLAG, HA, and 6*HIS into the desired gene loci. Researchers could use these tags to study the expression patterns, monitor the location changes and detect the expression level of the target protein.
We have templates for red, green, blue, yellow fluorescent protein tags, and you could find their coding sequences by clicking the direct download link below.
You could choose the sequence you want. If you have no idea, please contact us for suggestion.
The largest insertion SunyBiotech has ever done is currently 6kb in length.
Figure 1 Schematic diagram of precise gene insertion
|Price||Turnaround(weeks)||You need to provide||Deliverables|
|Starts from 1599 USD*||5-8|
*The price depends on the insertion size and difficulties. 1599 USD is for insertion smaller than 100bp. Contact us for a quote for larger sizes.
**If the precise insertion leads to embryonic lethal, larva arrest, or sterile phenotype in homozygotes, the precise heterozygote becomes the deliverable. If you want balanced heterozygotes, we could provide a Balancer Cross Service at a price of 400 USD.
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