Knock-In
Knock-In

Precise sequence knock-in

CRISPR/Cas9 sequence knock-in technology allows researchers to insert any sequence they want, such as GFP, mCherry, wrmScarlet, FLAG, HA, and 6*HIS into the desired gene loci. Researchers could use these tags to study the expression patterns, monitor the location changes and detect the expression level of the target protein. 

We have templates for red, green, blue, yellow fluorescent protein tags, and you could find their coding sequences by clicking the direct download link below. 

Coding sequence of green fluorescent protein.doc

Coding sequence of blue fluorescent protein.doc

Coding sequence of red fluorescent protein.doc

Coding sequence of yellow fluorescent protein.doc

You could choose the sequence you want. If you have no idea, please contact us for suggestion.

The largest insertion SunyBiotech has ever done is currently 6kb in length.

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Figure 1 Schematic diagram of precise gene insertion

PriceTurnaround(weeks)You need to provideDeliverables
Starts from 1399 USD*5-8
  1. 1. Name of gene on which you want to insert sequence
  2. 2. Detailed sequence you want to insert
  3. 3. The location of the insertion site
  4. 4. Strain on which you want to do this editing
  1. 1. Homozygous animals with precise sequence knock-in**
  2. 2. Sequencing report

*The price depends on the insertion size and difficulties. 1399 USD is for insertion smaller than 900bp. Contact us for a quote for larger sizes.

**If the precise deletion leads to embryonic lethal, larva arrest, or sterile phenotype in homozygotes, the precise heterozygote becomes the deliverable. If you want balanced heterozygotes, we could provide a Balancer Cross Service at a price of 400 USD.

How to Order:

To get a quote or place an order, please register and login your account, download and fill out the order form(s) of the service(s) you need, and email the form(s) to service@sunybiotech.com. We will send the confirmed order form with a quote to your email address (the one related to your account) after checking the information.

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Have Other Questions?

FAQs

You can always reach us at service@sunybiotech.com. Our customer service and editing experts will get back to you ASAP.