We are excited to announce that the CRISPR knock-in strains generated by SunyBiotech were cited in 《GENETICS》!
Membrane Fluidity Is Regulated Cell Nonautonomously by Caenorhabditis elegans PAQR-2 and Its Mammalian Homolog AdipoR2
This research was conducted by a team led by Dr. Marc Pilon, which mainly focused on how paqr-2 gene and its human homolog AsiproR2gene regulate the membrane homeostasis non-autonomously .
Figure 1 Updated model of membrane fluidity by PAQR-2/IGLR-2
The researchers used mosaic analysis for paqr-2 & iglr-2 and tissue-specific paqr-2 expression to show that membrane homeostasis is achieved cell nonautonomously. Specifically, they found that expression of paqr-2 in the hypodermis, gonad sheath cells, or intestine is sufficient to suppress systemic paqr-2 mutant phenotypes, including tail tip morphology, membrane fluidity in intestinal cells, cold and glucose intolerance, vitellogenin transport to the germline, germ cell development, and brood size.
Then, the research shows that the cell nonautonomous regulation of membrane homeostasis is conserved in human cells: HEK293 cells that express AdipoR2, a homolog of paqr-2, are able to normalize membrane fluidity in distant cells where AdipoR2 has been silenced.
Finally, the use of C. elegans mutants and small interfering RNA against D9 stearoyl-CoA desaturase in HEK293 cells indicates that D9 desaturases are essential for the cell nonautonomous maintenance of membrane fluidity.
They conclude that cells are able to share membrane components even when they are not in direct contact with each other, and that this contributes to the maintenance of membrane homeostasis in C. elegans and human cells.
In this research, PHX649 fat-6(syb649) mutant was generated by SunyBiotech. The syb649 is a CRISPR-modified fat-6allelewith GFP fused to the end of its coding region. The strain was used to study the function of paqr-2 function in the hypodermis.
Figure 2 Expression pattern of the fat-6::gfp
SunyBiotech is specializing in providing precise genome edited C. elegans. All strains generated by SunyBiotech will bare our nomenclature. PHX and syb is the strain designation and allele designation, respectively. We will continue working hard to provide high-quality services with higher efficiency.
Rakesh Bodhicharla, Ranjan Devkota, Mario Ruiz and Marc Pilon. Membrane Fluidity Is Regulated Cell Nonautonomously by Caenorhabditis elegans PAQR-2 and Its Mammalian Homolog AdipoR2. GENETICS September 1, 2018 vol. 210 no. 1 189-201;https://doi.org/10.1534/genetics.118.301272.